infB [2020-06-25 09:25:54]

translation initiation factor IF-2

Locus: BSU_16630

Isoelectric point: 5.19

Molecular weight: 78.35 kDa

Protein length: 716 aa

Gene length: 2151 bp

Function: translation

Product: initiation factor IF-2

Essential: yes

Synonyms

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Translation factors
• Information processing → Targets of second messengers → Targets of (p)ppGpp
• Groups of genes → Essential genes

Gene

Coordinates: 1,734,009 1,736,159

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Putative cellular metabolic sensor and regulator that oscillates between an active GTP-bound form under conditions allowing active protein syntheses and an inactive ppGpp-bound form when depleting of nutrients PubMed

Protein family

• TRAFAC class translation factor GTPase superfamily (according to UniProt)
• Classic translation factor GTPase family (according to UniProt)

Domains

• Nucleotide-binding domain that competitively binds GTP or ppGpp PubMed
• tr-type G domain (aa 217-386) (according to UniProt)

Effectors of protein activity

• GTP and ppGpp PubMed

Structure

• 1D1N (fMet-tRNA-fMet binding domain, Geobacillus stearothermophilus), 1Z9B (C1-subdomain, Geobacillus stearothermophilus)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: ylxS-nusA-ylxR-rplGA-infB-ylxP-rbfA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon

  Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • NusA: attenuation, NusA stimulates termination [Reference|http://www.nature.com/articles/nmicrobiol20157], in NusA regulon

  Regulation

  • autoregulation NusA reference
  • induced by glucose PubMed

References

Loading